Special ChBE Seminar: Dganit Danino
Thursday, August 4, 2011
2110 Chemical and Nuclear Engineering Bldg.
Professor Srinivasa Raghavan
Spatial and Temporal Organization of Soft Condensed Matter: Insight from Cryo-TEM
Presented by Dganit Danino
Department of Biotechnology and Food Engineering
TechnionIsrael Institute of Technology
Cryogenic-TEM has long been recognized as an instrumental method for studying the nanostructure of soft materials (e.g., surfactants, lipids, peptides and proteins) at high resolution. The method involves ultra-rapid cooling of liquid suspensions and creation of amorphous, vitrified specimens, which captures the native state of structured liquids thereby allowing to get directly detailed structural information at the nanoscale, as well as to explore self-organization mechanisms and dynamics.
I will briefly review the principles of the main cryo-TEM techniques, direct-imaging and freeze-fracture, and present from our recent studies examples relevant to nanoscience and nanotechnology, e.g., with micellar systems , peptide nanotubes [2, 3], DNA/lipid systems for gene therapy , and lipid and protein nanovehicles for oral delivery [5, 6].