ChBE Seminar Series: Steven M. Cramer
Tuesday, April 7, 2009
11:00 a.m.-12:15 p.m.
Room 2110, Chemical and Nuclear Engineering Bldg.
Professor F. Joseph Schork
Intelligent Design of Chemically Selective Displacers and Multimodal Chromatographic Systems Using Protein Libraries, NMR, SPR and Multi-Scale Simulations
Presented by Steven M. Cramer
William Weightman Walker Professor
Howard P. Isermann Department of Chemical and Biological Engineering
Rensselaer Polytechnic Institute
The design of novel multimodal chromatographic and chemically selective displacement systems requires a fundamental understanding of the binding of the ligands involved in these processes to protein surfaces. In this presentation a variety of experimental and theoretical approaches are employed to provide insight into the binding mechanisms involved in these processes and to exploit them for the design of chromatographic systems with unique selectivities. Studies were carried out with various combinations of protein libraries, stationary phase ligands and/or selective displacers. Protein libraries included protein charged ladders as well as homologous libraries of protein variants. Mixed mode ligands included a range of chemically diverse ligands and chemically selective displacers were selected from libraries designed for selective binding to specific protein classes. The tools employed in this work include high throughput screening, NMR, SPR and simulations. Nuclear Magnetic Resonance was conducted on a range of protein/stationary phase ligand or displacer mixtures to verify the binding to targeted proteins and the location of the binding event on both the ligand and protein surfaces. Surface plasmon resonance experiments were carried out to study the inherent kinetics of these processes. Molecular dynamic simulations were conducted to corroborate the NMR results and to provide further insight into the binding and preferred protein-binding orientations on the chromatographic surfaces. Finally, this data is integrated into multi-scale models to aid in the development of novel chromatographic processes with unique selectivities and high performance.
About the Speaker:
Steve Cramer is the William Weightman Walker Professor of Chemical and Biological Engineering at Rensselaer Polytechnic Institute, Troy, New York. He is conducting research on several areas related to chromatographic bioprocessing including: rational design of high affinity and chemically selective displacers for protein and oligonucleotide purification in ion exchange and hydrophobic displacement systems, multi-scale modeling of complex chromatographic behavior, molecular modeling of selectivity and affinity in chromatographic systems, spectroscopy/chromatographic investigation of protein-surface interactions, chromatography on a chip and novel separation systems for proteomics. He has won several awards including: the 2006 Alan S. Michaels Award for the Recovery of Biological Products (ACS Division of Biochemical Technology); School of Engineering Research Excellence Award, RPI; Presidential Young Investigator, National Science Foundation; and the Early Career Award, RPI. He has delivered major keynote and plenary addresses at a number of International Conferences, is a Fellow of the American Institute for Medical and Biological Engineering and is the Editor of the journal Separation Science and Technology.
This seminar is co-sponsored by the Fischell Department of Bioengineering.