ChBE Seminar Series: Jong-In Hahm
Tuesday, May 6, 2008
11:00 a.m.-12:00 p.m.
Room 2110 Chemical & Nuclear Engineering Bldg.
Professor Kyu Yong Choi
Engineering Nanomaterial Platforms: Toward Highly Sensitive and Specific Biomedical Detection
Presented by Jong-In Hahm
Penn State University
This talk presents an overview of our on-going nanomaterials research, aiming to provide more rapid, sensitive, and accurate detection of genetic and protein markers. Specifically, this talk will focus on the remarkably enhanced optical detection of DNA and proteins which is enabled by the use of nanoscale zinc oxide platforms.
Fluorescence detection is currently one of the most widely used methods in the areas of basic biological research, biotechnology, cellular imaging, medical testing, and drug discovery. Using model protein and nucleic acid systems, we demonstrate that engineered nanoscale zinc oxide nanostructures can significantly enhance the detection capability of biomolecular fluorescence. Without any chemical or biological amplification processes, nanoscale zinc oxide platforms enabled increased fluorescence detection of these biomolecules when compared to other commonly used substrates such as glass, quartz, polymer, and silicon. This ultrasensitive detection was due to the presence of ZnO nanomaterials which contributed greatly to the increased signal to noise ratio of biomolecular fluorescence.
We also demonstrate the easy integration potential of zinc oxide nanostructures into periodically patterned platforms which, in turn, will promote the assembly and fabrication of these materials into multiplexed, high-throughput, optical sensor arrays. These zinc oxide platforms will be extremely beneficial in accomplishing highly sensitive and specific detection of biological samples involving nucleic acids, proteins and cells, particularly under detection environments involving extremely small sample volumes of ultratrace-level concentrations.